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ID 31111
本文ファイル
著者
Ochiya, Takahiro
Ueda, Shinobu
Kominami, Yoko
Gon, Rina
Nishiki, Masayo
Hayashi, Masaomi
Sasaki, Atsushi
Shiraishi, Miho
Kashimoto, Naoki
Myojin, Yuki
キーワード
Differentiation
Hepatocyte
Transplantation
Stem cells
Liver
GFP
Transgenic rat
CCl4
NDC
医学
抄録(英)
While organ-specific stem cells with roles in tissue injury repair have been documented, their pathogenic significance in diseases and the factors potentially responsible for their activation remain largely unclear. In the present study, heart, kidney, brain, and skin samples from F344 transgenic rats carrying the GFP gene were transplanted into normal F344 rat liver one day after an intraperitoneal injection (i.p.) of carbon tetrachloride (CCl4) to test their differentiation capacity. The transplantation was carried out by female donors to male recipients, and vice versa. One week after transplantation, GFP antigen-positive cells with phenotypic characteristics of hepatocytes were noted. After two weeks, their extent increased, and at 4 weeks, large areas of strongly GFP-stained cells developed. All recipient livers had GFP antigen-positive hepatocyte cells. PCR analysis coupled with laser capture micro-dissection (LCM) revealed those cells to contain GFP DNA. Thus, our results indicate that tissue stem cells have multipotential ability, differentiating into hepatocytes when transplanted into an injured liver.
掲載誌名
Biochemical and Biophysical Research Communications
354巻
4号
開始ページ
841
終了ページ
845
出版年月日
2007-03-23
出版者
Elsevier
ISSN
0006-291X
NCID
出版者DOI
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
author
権利情報
Copyright (c) 2007 Elsevier Inc. All rights reserved.
関連情報URL
部局名
原爆放射線医科学研究所