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ID 112
本文ファイル
著者
Miyasako, Takanori
Kitayama, Shigeo
Dohi, Toshihiro
キーワード
IL-1
Voltage-dependent Ca2+ channel
P/Q-type Ca2+ channel
Catecholamine release
Ca2+
Adrenal chromaffin cells
NDC
医学
抄録(英)
Effects of interleukin (IL) on intracellular free Ca2+ concentration ([Ca2+]i) rise and catecholamine (CA) release were examined in isolated, cultured bovine adrenal chromaffin cells. IL-1α and IL-1β inhibited the rise of [Ca2+]i and CA release induced by acetylcholine (ACh) and excess KCl both in normal and in Ca2+-sucrose medium. IL-1 receptor antagonist, IL-1RA by pretreatment blocked the inhibitory actions of IL-1. IL-1α reduced CA release induced by veratridine in normal medium but not in the presence of diltiazem. Analysis using specific blockers for voltage-operated Ca2+ channels revealed that IL-1α specifically inhibited the P/Q-type Ca2+ channel to reduce [Ca2+]i rise induced by excess KCl. IL-1 did not affect [Ca2+]i rise induced either by bradykinin or caffeine in Ca2+- deprivated medium or via activation of store-operated Ca2+ channel. The inhibitory effects of IL-1 were blocked by pretreatments of cells with herbimycin A, U0126 and PD 98054, but not with SB202190, SP 600125 or pertussis toxin (PTX), inhibited the induction of the inhibitory action of IL-1.These results demonstrated that IL-1 inhibits stimulation-evoked [Ca2+]i rise and CA release in chromaffin cells by blocking voltage-operated P/O-type Ca2+ channels. The inhibitory action of IL-1 may be mediated through tyrosine kinase and MEK/ERK pathways.
掲載誌名
Biochimica et Biophysica Acta
1673巻
3号
開始ページ
160
終了ページ
169
出版年月日
2004-08-04
出版者
Elsevier B.V
ISSN
0304-4165
NCID
出版者DOI
PubMedID
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
author
権利情報
Copyright (c) 2004 Elsevier B.V
関連情報URL(IsVersionOf)
http://dx.doi.org/10.1016/j.bbagen.2004.04.012
部局名
医歯薬学総合研究科