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ID 109
本文ファイル
著者
Tamagawa, Hiroshi
Furuse, Mikio
Yoshitake, Yuka
Tsukita, Shoichiro
Itoh, Toshinori
Matsuda, Hikaru
Kiyono, Hiroshi
キーワード
claudin
follicle-associated epithelium
occludin
Peyer's patch
tight junction
NDC
医学
抄録(英)
Gut-associated lymphoreticular tissue (GALT), such as Peyer's patches (PP) and cecal patches, are important inductive sites for mucosal immune responses. As such, the GALT may have an epithelial barrier different from that of villous epithelium. In this study, we investigated the immunohistochemical distribution of the claudin family and occludin in the follicle-associated epithelium (FAE) of Peyer's patches and cecal patches of murine intestine. Unique profiles of claudin-2, -3, -4 and occludin expression were noted in the tight junctions of the FAE: claudin-4 was preferentially expressed in the apex region; claudin-2 was only weakly expressed on the crypt side of the FAE compared to stronger expression on the crypt side of villous epithelial cells; claudin-3 and occludin were found throughout the dome. These unique expression patterns were present also in cecal patch FAE. We also found that claudin-4 expression in the FAE of Peyer's patches and cecal patches corresponded with the presence of TUNEL-positive apoptotic cells, and Peyer's patch-deficient mice had expression patterns of claudin and occludin in villous epithelia similar to those in wild-type mice. We conclude that claudin-4 expression is preferentially associated with the dome region of FAE, the mucosal inductive site of the murine intestine. In that location and it might correlate with the cell life cycle, help maintain the apex configuration of the dome, or be a factor favoring the uptake of antigens by the FAE.
掲載誌名
Laboratory investigation
83巻
7号
開始ページ
1045
終了ページ
1053
出版年月日
2003-07
出版者
Nature Publishing Group
ISSN
0023-6837
出版者DOI
PubMedID
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
author
権利情報
Copyright (c) 2003 United States and Canadian Academy of Pathology
関連情報URL(IsVersionOf)
http://dx.doi.org/10.1097/01.LAB.0000078741.55670.6E
部局名
医歯薬学総合研究科