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ID 49948
本文ファイル
著者
HIRATA, Tatsumi
KAWAHARA, Akira
MUNEOKA, Yojiro
キーワード
Ganglion extract
Biological action
Catch muscle
NDC
医学
抄録(英)
Pedal ganglion homogenates from the bivalve, Mytilus edulis, were subjected to gel filtration and the biological activities of the extracts were assayed on the ABRM of the mussel. The extracts resolved into a catch-relaxing peak and a contraction-inhibiting peak of activities.
Relaxation of ACh-induced catch tension in the ABRM by catch-relaxing peak was not affected by pretreatment of the muscle with 10^-5 M FMRFamide, suggesting that the active principle of the peak is not FMRFamide. The relaxation was blocked by 5 x 10^-4 M mersalyl, which suggests that the active substance is neither dopamine nor octopamine. The relaxation was markedly depressed after the muscle had been denervated by treating it with KCl-EGTA solution, suggesting that the substance is not serotonin and that it relaxes the catch tension acting on intramuscular relaxing nerve elements. The relaxing activity of the peak was destroyed by incubating it with a protease, subtilisin. Thus, the active substance in the peak seems to be a peptide which acts presynaptically to increase the release of relaxing transmitter serotonin.
The contraction-inhibiting peak also lost its activity when incubated with subtilisin, suggesting that the inhibitory substance in the peak is also a peptide. The substance inhibited not only phasic contraction by repetitive electrical stimulation but also ACh contraction and FMRFamide contraction, which suggests that it acts directly on muscle fibres to inhibit the contractions.
掲載誌名
Hiroshima Journal of Medical Sciences
35巻
4号
開始ページ
397
終了ページ
402
出版年月日
1986-12
出版者
Hiroshima University School of Medicine
ISSN
0018-2052
NCID
PubMedID
言語
英語
NII資源タイプ
紀要論文
広大資料タイプ
学内刊行物(紀要等)
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
publisher
部局名
医歯薬学総合研究科
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