このエントリーをはてなブックマークに追加
ID 49942
本文ファイル
著者
SUMIDA, Hiroshi
AKIMOTO, Naotaka
LEE, Juin Yi
SATOW, Yukio
キーワード
Actin
Tannic acid
Phalloidin
Chick embryonic heart
NDC
医学
抄録(英)
Degree of binding reactivity to actin in variously fixed tissues was histochemically examined by using phalloidin and antiactin staining on paraffin or frozen sections of 5-day chick embryonic heart. Fluorescein-labeled phalloidin staining on frozen section of the specimens fixed with 4% formaldehyde showed intense staining of actin in the myocardium and the aorticopulmonary (AP) septum. However, phalloidin did not react to actin on paraffin sections of the specimens fixed with 4% formaldehyde, nor fixed with 4% formaldehyde and 1% tannic acid (TA). FITC-labeled antiactin staining on paraffin sections of the specimens fixed with 4% formaldehyde and 1% TA showed comparable fluorescent intensity with that of phalloidin staining on frozen sections. However antiactin staining on paraffin sections of the specimens fixed with 4% formaldehyde only showed reduction of fluorescent intensity around the Ap septum. Both phalloidin and antiactin react non-specifically to the tissues fixed with solutions including 1% glutaraldehyde. Since paraffin sections showed much better tissue preservation than frozen sections, we suggest that paraffin embedding with TA fixation is very useful for actin histochemistry using antiactin.
掲載誌名
Hiroshima Journal of Medical Sciences
35巻
4号
開始ページ
347
終了ページ
353
出版年月日
1986-12
出版者
Hiroshima University School of Medicine
ISSN
0018-2052
NCID
PubMedID
言語
英語
NII資源タイプ
紀要論文
広大資料タイプ
学内刊行物(紀要等)
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
publisher
部局名
医歯薬学総合研究科
他の一覧