このエントリーをはてなブックマークに追加
ID 14130
本文ファイル
著者
Foster, BL
Swanson, EC
Matsa-Dunn, D
Sato, S
Rutherford, RB
Somerman, MJ
キーワード
cementoblasts
phosphate
SIBLING
matrix metalloproteinase
osteopontin
NDC
医学
抄録(英)
Introduction: Cementoblasts, the cells responsible for tooth root cementum formation, are especially sensitive to local phosphate and pyrophosphate during development, as evidenced by cementum phenotypes resulting from altered phosphate/pyrophosphate distribution. SIBLING family members BSP, OPN, and DMP-1 are regulated by phosphate in cementoblasts and have been shown to activate three specific matrix metalloproteinase (MMP) partners: MMP2, MMP3, and MMP9, respectively, in vitro. The aim of this study was to examine regulatory effects of phosphate on SIBLING and MMP expression in cementoblasts, in vitro.
Materials & Methods: Immortalized murine cementoblasts were treated with inorganic phosphate, in vitro, and effects on gene expression (by real time RT-PCR and mouse total genome microarray) were observed. Dose-response 80.1-10 mM phosphate) and time-course (1-48hr) assays were performed. A sodium-phosphate uptake inhibitor, foscarnet, was used to better define phosphate-mediated effects on cells.
Results: Three SIBLING family members were regulated by phosphate: OPN (increased over 3000f control), DMP-1 (increased over 3,00027777701060f control), and BSP (decreased). MMP3 was dramatically increased (4,00026651125000f control), paralleling regulation of its partner OPN. Both MMP2 and MMP9 were slightly down-regulated. Time-course experiments indicated a response for SIBLING and MMP genes within 24 hr. Use of foscarnet demonstrated that phosphate uptake was required for observed changes in gene expression.
Discussion: These results indicate an effect of phosphate on cementoblast SIBLING and MMP expression in vitro. During cementum formation, phosphate may be an important regulator of cementoblast activity, including modulation of biomineralization, attachment, and matrix modification.
掲載誌名
Hiroshima Conference on Education and Science in Dentistry, 2006 : the 40th Anniversary of Hiroshima University Faculty of Dentistry
開始ページ
43
終了ページ
49
出版年月日
2006-01-08
言語
英語
NII資源タイプ
会議発表論文
広大資料タイプ
会議発表論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
publisher
関連情報URL(isPartOf)
http://ir.lib.hiroshima-u.ac.jp/meta-bin/mt-pdetail.cgi?smode=1&edm=0&tlang=1&cd=00014842
部局名
医歯薬学総合研究科