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ID 14208
本文ファイル
著者
Tomita, Kazunori
Kibe, Tatsuya
Kang, Ho-Young
Seo, Yeon-Soo
Uritani, Masahiro
Ushimaru, Takashi
NDC
生物科学・一般生物学
抄録(英)
It has been suggested that the Schizosaccharomyces pombe Rad50 (Rad50-Rad32-Nbs1) complex is required for the resection of the C-rich strand at telomere ends in taz1-d cells. However, the nuclease-deficient Rad32-D25A mutant can still resect the C-rich strand, suggesting the existence of a nuclease that resects the C-rich strand. Here, we demonstrate that a taz1-d dna2-2C double mutant lost the G-rich overhang at a semipermissive temperature. The amount of G-rich overhang in S phase in the dna2-C2 mutant was lower than that in wild-type cells at the semipermissive temperature. Dna2 bound to telomere DNA in a chromatin immunoprecipitation assay. Moreover, telomere length decreased with each generation after shift of the dna2-2C mutant to the semipermissive temperature. These results suggest that Dna2 is involved in the generation of G-rich overhangs in both wild-type cells and taz1-d cells. The dna2-C2 mutant was not gamma ray sensitive at the semipermissive temperature, suggesting that the ability to process double-strand break (DSB) ends was not affected in the dna2-C2 mutant. Our results reveal that DSB ends and telomere ends are processed by different mechanisms.
掲載誌名
Molecular and cellular biology
24巻
開始ページ
9557
終了ページ
9567
出版年月日
2004-11
出版者
American Society for Microbiology
ISSN
0270-7306
NCID
出版者DOI
PubMedID
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
author
権利情報
Copyright (c) American Society for Microbiology
関連情報URL(IsVersionOf)
http://dx.doi.org/10.1128/MCB.24.21.9557-9567.2004
部局名
先端物質科学研究科