このエントリーをはてなブックマークに追加
ID 48192
本文ファイル
著者
Nakano, Miyako 大学院先端物質科学研究科 広大研究者総覧
Mishra, Sushil K.
Tokoro, Yuko
Sato, Keiko
Nakajima, Kazuki
Yamaguchi, Yoshiki
Taniguchi, Naoyuki
Kizuka, Yasuhiko
キーワード
Glycomics
glycoprotein pathways
glycosylation
glycoproteins
glycoprotein structure
glycoproteomics
bisecting GlcNAc
fucosylation
GnT-III
HNK-1
sialylation
抄録(英)
Glycoproteins are decorated with complex glycans for protein functions. However, regulation mechanisms of complex glycan biosynthesis are largely unclear. Here we found that bisecting GlcNAc, a branching sugar residue in N-glycan, suppresses the biosynthesis of various types of terminal epitopes in N-glycans, including fucose, sialic acid and human natural killer-1. Expression of these epitopes in N-glycan was elevated in mice lacking the biosynthetic enzyme of bisecting GlcNAc, GnT-III, and was conversely suppressed by GnT-III overexpression in cells. Many glycosyltransferases for N-glycan terminals were revealed to prefer a nonbisected N-glycan as a substrate to its bisected counterpart, whereas no up-regulation of their mRNAs was found. This indicates that the elevated expression of the terminal N-glycan epitopes in GnT-III-deficient mice is attributed to the substrate specificity of the biosynthetic enzymes. Molecular dynamics simulations further confirmed that nonbisected glycans were preferentially accepted by those glycosyltransferases. These findings unveil a new regulation mechanism of protein N-glycosylation.
内容記述
This work was supported by Grant-in-Aid for Scientific Research (C) to Y.K. [17K07356], Leading Initiative for Excellent Young Researchers (LEADER) project to Y.K. from the Japan Society for the Promotion of Science (JSPS), by Takeda Science Foundation, and by Mochida Memorial Foundation for Medical and Pharmaceutical Research.
掲載誌名
Molecular & Cellular Proteomics
18巻
10号
開始ページ
2044
終了ページ
2057
出版年月日
2019-08-02
出版者
American Society for Biochemistry and Molecular Biology
ISSN
1535-9476
1535-9484
出版者DOI
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
author
権利情報
This research was originally published in Molecular & Cellular Proteomics. Miyako Nakano, Sushil K. Mishra, Yuko Tokoro, Keiko Sato, Kazuki Nakajima, Yoshiki Yamaguchi, Naoyuki Taniguchi, Yasuhiko Kizuka. Bisecting GlcNAc Is a General Suppressor of Terminal Modification of N-glycan. Mol Cell Proteomics. 2019; Vol 18: pp2044-pp2057. © the American Society for Biochemistry and Molecular Biology
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認、ご利用ください。
関連情報URL
部局名
先端物質科学研究科