Determination of Chemical-Specific IgGs in Serum by an Enzyme-Linked Immunosorbent Assay With Partial Peptides of Human Serum Albumin
JToxicolSci_43_25.pdf 675 KB
Vogel, Christoph F.A.
Many different types of chemicals are used in industry, and occupational allergies are becoming a serious problem in the field of industrial hygiene. In this study, we employed a novel enzyme-linked immunosorbent assay (ELISA) with partial peptides of human serum albumin (HSA) to quantify chemical-specific immunoglobulin G (IgG) in serum for evaluating exposure to chemicals. When HSA partial peptides containing lysine residues were mixed with formaldehyde (FA) or phthalic anhydride (PA), almost all lysine residues were lost. Mass spectrometry revealed that PA and FA formed imine and tertiary amine, respectively, with lysine residues in the peptides. Thus, we used FA- or PA-peptide adducts as an artificial antigen to detect FA- and PA-specific IgGs in serum. The concentrations of FA- and PA-specific IgGs in workers at plants utilizing plastic resins were significantly higher than those in general subjects. This method can estimate exposure levels to chemicals and thus be expected to contribute to the diagnosis of allergies in workers and to the prevention of health hazards due to harmful chemicals.
This work was partially supported by grants from the Ministry of Education, Culture, Sports, Science and Technology, Japan, KAKENHI for Y.I. and M.T. (Nos. 17HO4714 and 17KO9174), a grant from Hiroshima University Industry-Academia Collaboration Program for Y.I. and an Industrial Disease Clinical Research Grant to M.T. This manuscript has been checked by a professional language editing service(American Journal Experts).
The Journal of Toxicological Sciences
The Japanese Society of Toxicology
© 2018 The Japanese Society of Toxicology