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ID 48688
本文ファイル
著者
Ishihara, Yasuhiro 大学院総合科学研究科 広大研究者総覧
Ikeda-Ishihara, Nami
Koriyama, Chihaya
Kakiuchi, Noriaki
Tanaka, Masayuki
Vogel, Christoph F.A.
Kawamoto, Toshihiro
Tsuji, Mayumi
キーワード
Chemical-specific IgG
ELISA
Carbonyl compounds
抄録(英)
Many different types of chemicals are used in industry, and occupational allergies are becoming a serious problem in the field of industrial hygiene. In this study, we employed a novel enzyme-linked immunosorbent assay (ELISA) with partial peptides of human serum albumin (HSA) to quantify chemical-specific immunoglobulin G (IgG) in serum for evaluating exposure to chemicals. When HSA partial peptides containing lysine residues were mixed with formaldehyde (FA) or phthalic anhydride (PA), almost all lysine residues were lost. Mass spectrometry revealed that PA and FA formed imine and tertiary amine, respectively, with lysine residues in the peptides. Thus, we used FA- or PA-peptide adducts as an artificial antigen to detect FA- and PA-specific IgGs in serum. The concentrations of FA- and PA-specific IgGs in workers at plants utilizing plastic resins were significantly higher than those in general subjects. This method can estimate exposure levels to chemicals and thus be expected to contribute to the diagnosis of allergies in workers and to the prevention of health hazards due to harmful chemicals.
内容記述
This work was partially supported by grants from the Ministry of Education, Culture, Sports, Science and Technology, Japan, KAKENHI for Y.I. and M.T. (Nos. 17HO4714 and 17KO9174), a grant from Hiroshima University Industry-Academia Collaboration Program for Y.I. and an Industrial Disease Clinical Research Grant to M.T. This manuscript has been checked by a professional language editing service(American Journal Experts).
掲載誌名
The Journal of Toxicological Sciences
43巻
1号
開始ページ
25
終了ページ
31
出版年月日
2018
出版者
The Japanese Society of Toxicology
日本毒性学会
ISSN
0388-1350
出版者DOI
PubMedID
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
publisher
権利情報
© 2018 The Japanese Society of Toxicology
関連情報URL
部局名
総合科学研究科