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ID 25972
本文ファイル
著者
Kondo, Komei
Yamada, Keizo
Nakagawa, Ayami
Morikawa, Hiromichi
キーワード
Germin-like protein
GLP
nitrogen dioxide
nitric oxide
reactive nitrogen species
superoxide dismutase
reactive oxygen species
NDC
生物科学・一般生物学
抄録(英)
Atmospheric nitrogen dioxide (NO2) is an environmental oxidant that is removed through direct uptake by foliage, but plant responses to this highly reactive gas are not well understood at the molecular level. From NO2-exposed leaves of a woody azalea (Rhododendron mucronatum), we cloned two cDNAs (RmGLP1 and RmGLP2) for germin-like proteins (GLPs), a group of ubiquitous plant proteins that have been implicated in various plant physiological and developmental processes. Quantitative analysis of mRNA expression, together with immunoblotting data, showed that foliar exposure to NO2 caused a robust induction of these GLP-encoding genes. When produced in tobacco cell culture, recombinant RmGLP2 was secreted into the apoplast, where it exhibited superoxide dismutase activity. RmGLP1 and RmGLP2 represent the first examples of plant genes that are responsive to airborne NO2. These enzymes might have a potential role in extracellular defense mechanisms through attenuation of interactions between reactive nitrogen and oxygen species.
掲載誌名
Biochemical and Biophysical Research Communications
377巻
3号
開始ページ
857
終了ページ
861
出版年月日
2008-12-18
出版者
Academic Press Inc.
Elsevier Science
ISSN
0006-291X
NCID
出版者DOI
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
author
権利情報
Copyright (c) 2008 Elsevier Inc.
関連情報URL
部局名
理学研究科