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ID 20760
本文ファイル
著者
Taniguchi, Koji
Nomura, Kazutaka
Hata, Yumehiro
Nishimura, Tomoki
Asami, Yasuo
キーワード
silicon device
protein targeting
silica
ribosomal protein L2
protein array
protein orientation
green fluorescent protein
luciferase
NDC
化学
抄録(英)
Targeting functional proteins to specific sites on a silicon device is essential for the development of new biosensors and supramolecular assemblies. Using intracellular lysates of several bacterial strains, we found that ribosomal protein L2 binds tightly to silicon particles, which have surfaces that are oxidized to silica. A fusion of E. coli L2 and green fluorescence protein adsorbed to the silica particles with a Kd of 0.7 nM at pH 7.5 and also adsorbed to glass slides. This fusion protein was retained on the glass slide even after washing for 24 h with a buffer containing 1 M NaCl. We mapped the silica-binding domains of E. coli L2 to amino acids 1-60 and 203-273. These two regions seemed to cooperatively mediate the strong silica-binding characteristics of L2. A fusion of L2 and firefly luciferase also adsorbed on the glass slide. This L2 silica-binding tag, which we call the "Si-tag", can be used for one-step targeting of functional proteins on silica surfaces.
掲載誌名
Biotechnology and Bioengineering
96巻
6号
開始ページ
1023
終了ページ
1029
出版年月日
2007
出版者
John Wiley & Sons, Inc.
ISSN
0006-3592
NCID
出版者DOI
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
author
権利情報
Copyright (c) 2006 Wiley Periodicals, Inc.
関連情報URL
部局名
先端物質科学研究科