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ID 25967
本文ファイル
著者
Satoh, Tetsuya
Shinoda, Yasuharu
Alexandrov, Maxym
キーワード
Bioluminescence assay
ATP amplification
Continuous-flow system
NDC
生物科学・一般生物学
抄録(英)
We constructed a novel ATP amplification reactor using a continuous-flow system, and this allowed us to increase the sensitivity of quantitative bioluminescence assay by controlling the number of ATP amplification cycles. We previously developed a bioluminescence assay coupled with ATP amplification using a batch system. However, it was difficult to control the number of amplification cycles. In this study, ATP amplification was performed using a continuous-flow system, and significant linear correlations between amplified luminescence and initial ATP concentration were observed. When performing 4 cycles of continuous-flow ATP amplification, the gradient of amplification was 1.87N. Whereas lower quantifiable level was 500 pM without amplification, values as low as 50 pM ATP could be measured after amplification. Sensitivity thus increased 10-fold, with further improvements expected with additional amplification cycles. The continuous-flow system thus effectively increased the sensitivity of quantitative bioluminescence assay.
掲載誌名
Analytical Biochemistry
379巻
1号
開始ページ
116
終了ページ
120
出版年月日
2008-08-01
出版者
Academic Press Inc.
Elsevier Science
ISSN
0003-2697
NCID
出版者DOI
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
author
権利情報
Copyright (c) 2008 Elsevier Inc.
関連情報URL
部局名
先端物質科学研究科