このエントリーをはてなブックマークに追加
ID 14282
本文ファイル
別タイトル
インターロイキン1αは線維芽細胞依存的にマスト細胞の増殖を促進する
著者
亀好 良一
NDC
医学
抄録(英)
Mast cell hyperplasia is observed in variousinflammatory skin diseases. Although the pathogenesis of these conditions remains largely uninvestigated, it is speculated that lesional mediators provide favorable microenvironment for mast cell growth. We have investigated the effect of an inflammatory cytokine, IL-1, on mast cell growth in a mast cell/fibroblast co-culture system. When mouse bone marrow-derived cultured mast cells (BMMC) were cultured on a NIH/3T3 fibroblast monolayer, IL-1 stimulated mast cell proliferation. However, IL-1did not stimulate 3H-thymidine incorporation in BMMC in the absence of fibroblasts. Separation of BMMC from fibroblasts by a permeable micropore membrane reduced the effect of IL-1. When BMMC were prepared from W/Wv-mice, which lack functional c-kit, or when NIH/3T3 fibroblasts were substituted with Sl/Sld-derived fibroblasts, which lack membrane-bound stem cell factor (SCF), a lower, but significant, effect of IL-1 was observed. Flow cytometric analysis revealed no enhancement of SCF expression on fibroblasts by IL-1. Neutralizing antibodies against IL-3, IL-4, IL-10, and nerve growth factor (NGF) showed no inhibition. On the other hand, indomethacin inhibited the effect of IL-1, and prostaglandin E2 induced mast cell growth in the co-culture. These results indicate that IL-1 stimulates mast cell growth by a fibroblast-dependent mechanism, where SCF/c-kit interaction may participate in a major way. The mast cell growth activity induced by this cytokine is, at least in part, attributed to prostaglandins. Inflammatory cytokines may thus contribute to mast cell hyperplasia in skin diseases.
出版者
Hiroshima University
言語
英語
NII資源タイプ
学位論文
広大資料タイプ
学位論文
DCMIタイプ
text
フォーマット
application/pdf
権利情報
Copyright (c) 2000 by Author
学位記番号
乙第3383号
授与大学
広島大学(Hiroshima University)
学位名
博士(医学)
学位名の英名
Medicine
学位の種類の英名
doctoral
学位授与年月日
2000-06-22
部局名
医歯薬学総合研究科