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ID 33079
本文ファイル
別タイトル
Artifi cial rearing and oviposition of Waminoa sp. (Acoela, Acoelomorpha) : Toward the development of a model system to study animal-algal symbiosis. <Articles>
著者
彦坂(片山) 智恵
キーワード
Symbiosis
Acoelomorpha
Symbiodinium
Amphidinium
oogenesis
oviposition
NDC
生物科学・一般生物学
抄録(英)
Waminoa sp. is an acoelomorph worm that infests corals and harbors two species of dinofl agellates, Amphidinium sp. and Symbiodinium sp.. These symbiotic algae are inherited vertically from parent to offspring during oogenesis. In this study, we established a method for artificial breeding of Waminoa sp., as the first step toward using Waminoa sp. in the study of the mechanism underlying animal–algal symbiosis and the evolution of this symbiosis. We developed a system in which an aquarium circulated approximately 80 L of artifi cial seawater between an upper aquarium and a lower sump, each of which was 45 cm × 30 cm × 35 cm in size and contained 40 L of seawater. Water flowed into the upper aquarium from the sump alternately through a pipe outlet. The upper aquarium contained the hard coral Symphyllia valenciennesii, live rocks, and a layer of fine sand. A 150-W metal halide lamp was suspended over the aquarium to aid photosynthesis. The system was maintained at a salinity of 32–33 ppt and a temperature of 23°C, on a 14-h light (17–50 μmol quanta m-2s-1) : 10-h dark photoperiod. We succeeded in producing almost every stage of the life cycle of Waminoa sp., including the zygote, embryo, larva, juvenile, and adult stages, independent of seasons. In addition, we developed an artificial oviposition procedure that involved isolation from coral in the mature stage. The system developed in this study will serve as a foundation for future studies on acoel–algal symbiosis.
掲載誌名
広島大学大学院総合科学研究科紀要. I, 人間科学研究
5巻
開始ページ
39
終了ページ
46
出版年月日
2010-12-31
出版者
広島大学大学院総合科学研究科
ISSN
1881-7688
NCID
SelfDOI
言語
日本語
NII資源タイプ
紀要論文
広大資料タイプ
学内刊行物(紀要等)
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
publisher
権利情報
Copyright (c) 2010 by Graduate School of Integrated Arts and Sciences, Hiroshima University All rights reserved
部局名
総合科学研究科
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