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ID 37681
本文ファイル
著者
Horie, Shigeaki
Yasuda, Mineo
キーワード
Retinoic acid
Palatal ruga
Mouse
Cleft palate
NDC
医学
抄録(英)
Pregnant Jcl:ICR mice were orally given all-trans-retinoic acid (RA) at dose levels from 0.08 to. 80 mg/kg once at days 10.5, 11.5, or 12.5 (vaginal plug = day 0) of gestation. The dams were sacrificed at day 18.5 of gestation, and the fetuses were dissected. After fixation in Bouin's solution, the fetal palates were observed under a dissecting microscope. Pattern alterations rare in the control fetuses were shortness, fusion, maldirection, trirugal malalignment, modified cross, and cross. These alterations were defined as abnormalities. Cleft palate was induced at dose levels of 20 mg/kg and above. At each treatment day, the total incidence of abnormal rugae increased from the dose of 0.3 or 0.6 mg/kg, dose-dependently. Although missing ruga-8 was a common pattern alteration in vehicle controls, its incidence increased dose-dependently. The incidence of supernumerary posterior to ruga 3 increased dose-dependently after treatment at day 11.5 or day 12.5 of gestation, however the increase was not observed after treatment at day 10.5 of gestation. These findings indicate that the abnormalities of ruga, missing ruga-8 and supernumerary posterior to ruga 3 are very sensitive indicators of teratogenicity of RA.
内容記述
This work was supported by a grant from the Ministry of Health and Welfare of Japan.
掲載誌名
Hiroshima Journal of Medical Sciences
50巻
1号
開始ページ
17
終了ページ
25
出版年月日
2001-03
出版者
Hiroshima University Medical Press
ISSN
0018-2052
NCID
言語
英語
NII資源タイプ
紀要論文
広大資料タイプ
学内刊行物(紀要等)
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
publisher
部局名
医歯薬学総合研究科
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