このエントリーをはてなブックマークに追加
ID 37943
本文ファイル
著者
Yakehiro, Masuhide
キーワード
Sodium channel
Polyamine
Frog ventricular cells
Block
NDC
医学
抄録(英)
The mechanism underlying the Na channel blocking action of guanidyl-side armed cyclam (G-cyclam) was studied using conventional patch-clamp methods. G-cyclam applied to the cytoplasmic surface of the membrane reduced the amplitude of single Na channel currents without inducing a flickering block. This effect was enhanced by depolarization and was fully reversible upon washout of the drug. The relationship between the concentration of G-cyclam and the reduction of unitary current could be expressed mathematically assuming one-to-one stoichiometry. During maximal suppression of the single channel current by G-cyclam approximately 40% of the current remained. Low concentration of G-cyclam (3×10-4M) prolonged, while higher concentration (3×10-3M) shortened the mean open time suggesting the involvement of two processes in the Na channel blocking action of this agent. It appears that low concentration of G-cyclam induce rapid and frequent transition between open and less conductive state resulting in a reduced current, and higher concentration make the channel nonconductive with slower single channel kinetics.
内容記述
This work was supported in part by a Grant-in-Aid from the Ministry of Education, Science and Culture of Japan and a grant from the Tsuchiya Foundation.
掲載誌名
Hiroshima Journal of Medical Sciences
41巻
3号
開始ページ
49
終了ページ
55
出版年月日
1992-09
出版者
Hiroshima University Medical Press
ISSN
0018-2052
NCID
PubMedID
言語
英語
NII資源タイプ
紀要論文
広大資料タイプ
学内刊行物(紀要等)
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
publisher
部局名
医歯薬学総合研究科
他の一覧