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ID 37934
本文ファイル
著者
Asano, Kenji
Iwasaki, Hiroya
Fujimura, Katsuyuki
Ikeda, Mitsunori
Sugimoto, Yoshimi
Matsubara, Akemi
Yano, Kazuko
Irisawa, Hiroko
Kono, Fujiko
Kanbe, Masayuki
Tsubokura, Tokuo
Inouye, Yoshio
Nakamura, Shoshiro
キーワード
Creatinine
Creatinine amidohydrolase
Creatine amidinohydrolase
Sarcosine oxidase
NDC
医学
抄録(英)
Hydrogen peroxide was generated from creatinine by the sequential enzyme reactions of creatinine amidohydrolase, creatine amidinohydrolase and sarcosine oxidase. Hydrogen peroxide was, in turn, used stoichiometrically for the condensation of 4-aminoantipyrine and N-ethyl-N-(2-hydroxy-3-sulfopropyl)-m-toluidine catalyzed by horse-radish peroxidase, resulting in the formation of quinone dye with maximum absorbance at 546 nm. The optimized assay conditions for the enzymatic determination of creatinine in a HITACHI 7250 autoanalyzer was established. This system, which requires less than 5 μl of sample, was found to be the most economical for laboratories equipped with autoanalyzers.
掲載誌名
Hiroshima Journal of Medical Sciences
41巻
1号
開始ページ
1
終了ページ
5
出版年月日
1992-03
出版者
Hiroshima University Medical Press
ISSN
0018-2052
NCID
PubMedID
言語
英語
NII資源タイプ
紀要論文
広大資料タイプ
学内刊行物(紀要等)
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
publisher
部局名
医歯薬学総合研究科
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