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ID 38142
本文ファイル
著者
Yoshimoto, Kanji
Komura, Setsuo
Fujita, Tomio
キーワード
Brain dialysis
Dopamine
Serotonin
NDC
医学
抄録(英)
The present study demonstrated that it is possible to measure the endogenous extracellular dopamine (DA) and its metabolite dihydroxyphenylacetic acid (DOPAC), endogenous extracellular 5-hydroxytryptamine (5HT), its metabolites 5-hydroxyindoleacetic acid (5HIAA), 5-hydroxytryptophol (5HTOL) and its precursor 5-hydroxytryptophan (5HTP) simultaneously from the striatum of freely moving rats using intracerebral dialysis combined with high performance liquid chromatography (HPLC) with electrochemical detector (ECD).

Basal extracellular levels of DA, DOPAC, 5HTP, 5HT, 5HIAA and 5HTOL in the striatum were estimated to be 18.89 ±. 2.23 (pg/25 μl/60 min), 7.25 ± 0.32 (ng/25 μl/60 min), 13.35 ± 3.01 (pg/25 μl/60 min), 5.32 ± 0.92 (pg/25 μl/60 min), 2.41 ± 0.08 (ng/25 μl/60 min) and 7.69 ± 0.45 (pg/25 μl/60 min), respectively. Pargyline (75 mg/kg i.p.) and tryptophan (100 mg/kg i.p.) increased the 5HT level in the striatum perfusates by 3-fold, whereas DOPAC and 5HIAA levels decreased by approximately 20% and 8%, respectively. In addition, DA and 5HTOL levels were not changed.

This method is suitable for monitoring dopaminergic and serotonergic activities dynamically.
掲載誌名
Hiroshima Journal of Medical Sciences
37巻
1号
開始ページ
41
終了ページ
43
出版年月日
1988-03
出版者
Hiroshima University Medical Press
ISSN
0018-2052
NCID
PubMedID
言語
英語
NII資源タイプ
紀要論文
広大資料タイプ
学内刊行物(紀要等)
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
publisher
部局名
医歯薬学総合研究科
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