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ID 14206
本文ファイル
著者
Nakazaki, Tomofumi
Akamatsu, Yufuko
Watanabe, Kikuo
Tomita, Kazunori
Lindsay, Howard D.
Shinagawa, Hideo
Iwasaki, Hiroshi
キーワード
MRN
nbs1
methyl methanesulfonate
genetic disease
Nijmegen breakage syndrome
NDC
物理学
抄録(英)
The human MRN complex is a multisubunit nuclease that is composed of Mre11, Rad50, and Nbs1 and is involved in homologous recombination and DNA damage checkpoints. Mutations of the MRN genes cause genetic disorders such as Nijmegen breakage syndrome. Here we identified a Schizosaccharomyces pombe nbs1+ homologue by screening for mutants with mutations that caused methyl methanesulfonate (MMS) sensitivity and were synthetically lethal with the rad2△ mutation. Nbs1 physically interacts with the C-terminal half of Rad32, the Schizosaccharomyces pombe Mre11 homologue, in a yeast two-hybrid assay. nbs1 mutants showed sensitivities to γ-rays, UV, MMS, and hydroxyurea and displayed telomere shortening similar to the characteristics of rad32 and rad50 mutants. nbs1, rad32, and rad50 mutant cells were elongated and exhibited abnormal nuclear morphology. These findings indicate that S. pombe Nbs1 forms a complex with Rad32-Rad50 and is required for homologous recombination repair, telomere length regulation, and the maintenance of chromatin structure. Amino acid sequence features and some characteristics of the DNA repair function suggest that the S. pombe Rad32-Rad50-Nbs1 complex has functional similarity to the corresponding MRN complexes of higher eukaryotes. Therefore, S. pombe Nbs1 will provide an additional model system for studying the molecular function of the MRN complex associated with genetic diseases.
掲載誌名
Molecular and cellular biology
23巻
開始ページ
6553
終了ページ
6563
出版年月日
2003-09
出版者
American Society for Microbiology
ISSN
0270-7306
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
author
権利情報
Copyright (c) 2003 American Society for Microbiology
関連情報URL(IsVersionOf)
http://dx.doi.org/10.1128/MCB.23.18.6553-6563.2003
部局名
先端物質科学研究科