このエントリーをはてなブックマークに追加
ID 14657
本文ファイル
著者
Baes, Aloysius U.
Okada, Mitsumasa
キーワード
coagulation
purification
isolation
characterization
moringa olelfera
NDC
公害・環境工学
内容記述
It is known that M. oleifera contains a natural coagulant in the seeds. In our previous research, the method using salt water to extract the active coagulation component from M. oleifera seeds was developed and compared with the conventional method using water. In this research, the active coagulation component was purified from a NaCl solution crude extract of Moringa oleifera seeds. The active component was isolated and purified from the crude extract through a sequence of steps that included salting-out by dialysis, removal of lipids and carbohydrates by homogenization with acetone, and anion exchange. Specific coagulation activity of the active material increased up to 34 times more than the crude extract after the ion exchange. The active component was not the same as that of water extract. The molecular weight was about 3000 Da. The Lowry method and the phenol-sulfuric acid method indicated that the active component was neither protein nor polysaccharide. The optimum pH of the purified active component for coagulation of turbidity was pH 8 and above. Different from the conventional water extracts, the active component can be used for waters with low turbidity without increase in the dissolved organic carbon concentration.
掲載誌名
Water Research
35巻
2号
開始ページ
405
終了ページ
410
出版年月日
2001-02
出版者
Elsevier
ISSN
0043-1354
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
author
権利情報
Copyright (c) 2000 Elsevier Science Ltd.
関連情報URL(IsVersionOf)
http://dx.doi.org/10.1016/S0043-1354(00)00290-6
部局名
工学研究科