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ID 30803
本文ファイル
著者
Makihira, Seicho
Mine, Yuichi
Shuto, Takahiro
Iwata, Satoshi
Hosokawa, Ryuji
Kamoi, Kohei
Okazaki, Shota
Yamaguchi, Yu
キーワード
Titanium ion
Cell viability
TLR-2
TLR-4
Gingival epithelial cells
NDC
医学
抄録(英)
Gingival epithelial-like cells (GE-1) were cultured and used to examine the cellular responses of gingival tissues to varying concentrations of titanium (Ti) ions. Titanium ions at concentrations of more than 13 ppm significantly decreased the viability of GE-1 cells and increased LDH release from the cells into the supernatant, but had no significant effect on their caspase 3 activity. These data suggest that a high concentration of Ti ions induced necrosis of the GE-1 cells. Titanium ions at a concentration of 5 ppm significantly increased the level of CCL2 mRNA expression in GE-1 cells exposed to lipopolysaccharide derived from Porphyromonas gingivalis in a synergistic manner. Moreover, the mRNA expression levels of TLR-4 and ICAM-1 in GE-1 cells loaded with Ti ions at 9 ppm were significantly enhanced as compared with those in GE-1 cells without Ti stimulation. We suggest that Ti ions are in part responsible for monocyte infiltration in the oral cavity by elevating the sensitivity of gingival epithelial cells to microorganisms. Taken together, these data indicate that Ti ions may be involved in cytotoxicity and inflammation at the interfaces of dental implants and gingival tissue.
掲載誌名
Toxicology in Vitro
24巻
7号
開始ページ
1905
終了ページ
1910
出版年月日
2010-10
出版者
Pergamon Elsevier Science Ltd
ISSN
0887-2333
NCID
出版者DOI
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
author
権利情報
Copyright (c) 2010 Elsevier Ltd
関連情報URL
部局名
医歯薬学総合研究科