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ID 25653
本文ファイル
著者
Genmoto, K.
Nishizawa, Toyohiko
Muroga, Kiyokuni
キーワード
Vibrio penaeicida
rRNA
PCR Kuruma prawn
Vibriosis
NDC
動物学
抄録(英)
Vibrio penaeicida is the causative bacterium of vibriosis in cultured kuruma prawn Penaeus japonicus in Japan. To develop a specific and sensitive method for the detection of the pathogen, a species-specific sequence in the 16S rRNA of V. penaeicida was determined and a polymerase chain reaction (PCR)-based method was devised on the basis of the sequence. Prior to sequencing, a part of the variable regions of the 16S rRNA was amplified by using primers designed from 2 conserved regions according to previously reported data on Vibrionaceae. The region of the 16S rRNA (nucleotide numbers 440 to 490 in Escherichia coli 16S rRNA) obtained by this procedure was found to be species-specific for V. penaeicida. It was confirmed that PCR and RT (reverse transcription)-PCR amplifications with a sense primer designed from the V. penaeicida-specific sequence were both able to differentiate V. penaeicida from other prawn-pathogenic vibrios. 16S rRNA-targeted RT-PCR was demonstrated to have 100 times higher sensitivity than 16S rDNA-targeted PCR and 10 fg of total nucleic acids extracted from cultured bacterial cells was sufficient to yield the visible fragment in gel electrophoresis. These results indicate that RT-PCR amplification with this primer is useful for specific and sensitive detection of V. penaeicida.
掲載誌名
Diseases of Aquatic Organisms
24巻
3号
開始ページ
185
終了ページ
189
出版年月日
1996-03-07
出版者
Inter-Research
ISSN
0177-5103
1616-1580
NCID
出版者DOI
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
publisher
権利情報
Copyright (c) 1996 Inter-Research.
関連情報URL
部局名
生物圏科学研究科