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ID 20788
本文ファイル
著者
Fujimoto, Kenta
キーワード
Gene duplication
Matrix metalloproteinase-9
Metamorphosis
Programmed cell death
Thyroid hormone
NDC
動物学
抄録(英)
The drastic morphological changes of the tadpole are induced during the climax of anuran metamorphosis, when the concentration of endogenous thyroid hormone is maximal. The tadpole tail, which is twice as long as the body, shortens rapidly and disappears completely in several days. We isolated a cDNA clone, designated as Xl MMP-9TH, similar to the previously reported Xenopus laevis MMP-9 gene, and showed that their Xenopus tropicalis counterparts are located tandemly about 9 kb apart from each other in the genome. The Xenopus MMP-9TH gene was expressed in the regressing tail and gills and the remodeling intestine and central nervous system, and induced in thyroid hormone-treated tail-derived myoblastic cultured cells, while MMP-9 mRNA was detected in embryos. Three thyroid hormone response elements in the distal promoter and the first intron were involved in the upregulation of the Xl MMP-9TH gene by thyroid hormone in transient expression assays, and their relative positions are conserved between X. laevis and X. tropicalis promoters. These data strongly suggest that the MMP-9 gene was duplicated, and differentiated into two genes, one of which was specialized in a common ancestor of X. laevis and X. tropicalis to be expressed in degenerating and remodeling organs as a response to thyroid hormone during metamorphosis.
掲載誌名
Development Growth and Differentiation
48巻
4号
開始ページ
223
終了ページ
241
出版年月日
2006-05
出版者
Blackwell
ISSN
0012-1592
NCID
出版者DOI
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
author
権利情報
Copyright (c) 2006 Authors, The definitive version is available at www.blackwell-synergy.com
関連情報URL(IsVersionOf)
http://dx.doi.org/10.1111/j.1440-169X.2006.00859.x
部局名
理学研究科