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ID 20659
本文ファイル
著者
Fujimoto, Kenta
キーワード
amphibian metamorphosis
aatrix metalloproteinases
thyroid hormone response element
thyroid hormone
xenopus
NDC
生物科学・一般生物学
抄録(英)
Several matrix metalloproteinases (MMP) are induced by thyroid hormone (TH) during the climax of amphibian metamorphosis, and play a pivotal role in the remodeling intestine and the regressing tail and gills by degrading the extracellular matrix (ECM). We compared MMP gene expression levels precisely by the quantitative real time reverse transcription-polymerase chain reaction. The expression of MMP genes increases prominently at NF stages 60, 60-61, and 62 in the intestine, gills, and tail, respectively, when the drastic morphological change starts in each organ. The gene expression analysis in the TH-treated tadpoles and cell line revealed that MMP mRNAs are up-regulated in response to TH quickly within several hours to low levels and then increase in a day to high levels. All TH-induced MMP genes have thyroid hormone response elements (TREs). The presence of high affinity TREs in MMP genes correlates with the early TH-induction. Based on these results, we propose that TH stimulates the transcription of MMP genes through TREs within several hours to low levels, and then bring about the main increase of mRNAs by TH-induced transcriptional factors including TH receptor β in cell type-specific transcriptional environment.
掲載誌名
Development, Growth & Differentiation
49巻
2号
開始ページ
131
終了ページ
143
出版年月日
2007-02
出版者
Blackwell Publishing
ISSN
0012-1592
NCID
出版者DOI
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
author
権利情報
Copyright (c) 2007 Blackwell Publishing. The definitive version is available at www.blackwell-synergy.com
関連情報URL
部局名
理学研究科