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ID 20570
本文ファイル
著者
Nokihara, Kiyoshi
Yasuhara, Tadashi
Lerner, Ethan A.
Wray, Victor
キーワード
vasodilator
maxadilan
PAC1 receptor
disulfide isomer
highly efficient solid-phase synthesis
difficult sequence
melanophore assay
NDC
医学
抄録(英)
A potent and persistent non-mammalian derived vasodilator, maxadilan (Maxa) consists of 61 amino acids with two disulfide linkages and acts as an agonist of the type I receptor of pituitary adenylate cyclase activating polypeptide (PACAP), although there is very little sequence similarity. The total chemical syntheses of Maxa, its disulfide isomers and various fragments have been performed successfully by highly efficient solid-phase peptide synthesis (SPPS). A "difficult sequence", envisaged in the middle region of Maxa, could be overcome by improved synthesis protocols. After assembly peptides were liberated from the resin by cleavage. Peptides having disulfide(s) were purified by two steps of preparative HPLC using cation exchange followed by reverse phase columns. Purified peptides were characterized by HPLC, Edman-sequencing, amino acid analysis and mass spectrometry in addition to disulfide form determination. The peptides obtained were used for recognition studies by the melanophore assay to confirm the native disulfide form. Peptide libraries related to Maxa, produced in the present study, will be useful for the elucidation of the structural requirements of Maxa for interaction with the PACAP type 1 receptor (PAC1).
掲載誌名
International Journal of Peptide Research and Therapeutics
13巻
1-2号
開始ページ
377
終了ページ
386
出版年月日
2007-06
出版者
Springer
ISSN
1573-3149
NCID
出版者DOI
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
author
権利情報
Copyright (c) 2007 Springer "The original publication is available at www.springerlin.com"
関連情報URL
部局名
医歯薬学総合研究科