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ID 17056
本文ファイル
著者
Kawakami, Satomi
Kashiwagi, Keiko
Yamashita, Masamichi
Kashiwagi, Akihiko
キーワード
Space environments
Hypergravity
Xenopus laevis
Amphibian development
Gene expression
Apoptosis
Developmental retardation
Morphological abnormalities
NDC
生物科学・一般生物学
抄録(英)
This study investigates how rearing under conditions of hypergravity affects amphibian development, Xotx2 and Xag1 gene expression and apoptosis. Uncleaved Xenopus laevis eggs 20 min after insemination, 2 cell stage embryos, and gastrula stage embryos were raised at 2G and 5G, while controls were raised in normal gravity. Apoptosis in brain and eye inner structures of hatching embryos was scored using the TUNEL staining method, and gene expression in tail-bud embryos was analyzed by whole-mount in situ hybridization. Results showed that: (1) 5G retarded the development of eggs and embryos and induced microcephaly and microphthalmia. (2) 5G suppressed the expression of the two genes, Xotx2 (involved in fore- and midbrain and eye development) and Xag1 (regulating cement gland formation). (3) Eggs and 2 cell stage embryos raised at 5G showed a greater extent of brain and eye apoptosis compared with controls, while those raised at 2G showed no significant difference. These findings suggest that high gravity suppresses certain gene functions and induces abnormal apoptosis in brain and eyes, resulting in developmental retardation and various morphological abnormalities.
掲載誌名
Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology
145巻
1号
開始ページ
65
終了ページ
72
出版年月日
2006-09
出版者
Elsevier
ISSN
1095-6433
NCID
出版者DOI
PubMedID
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
author
権利情報
Copyright (c) 2006 Elsevier Ltd.
関連情報URL
部局名
理学研究科