Characteristics of High-Molecular-Weight Hyaluronic Acid as a Brain-Derived Neurotrophic Factor Scaffold in Periodontal Tissue Regeneration
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ID | 32031 |
本文ファイル | |
著者 |
Sakai, Noriyuki
Nagahara, Takayoshi
Kawaguchi, Hiroyuki
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NDC |
医学
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抄録(英) | Brain-derived neurotrophic factor (BDNF), for which bovine collagen-derived atelocollagen is used as a scaffold, enhances periodontal tissue regeneration. However, a scaffold that does not contain unknown ingredients is preferable. Since the synthesized high-molecular-weight (HMW)-hyaluronic acid (HA) is safe and inexpensive, we evaluated the efficacy of HMW-HA as a BDNF scaffold. CD44, a major receptor of HA, was expressed in cultures of human periodontal ligament cells, and HMW-HA promoted the adhesion and proliferation of human periodontal ligament cells, although it did not influence the mRNA expression of bone (cementum)-related proteins. The in vitro release kinetics of BDNF from HMW-HA showed that BDNF release was sustained for 14 days. Subsequently, we examined the effect of BDNF/HMW-HA complex on periodontal tissue regeneration in dogs. A greater volume of newly formed alveolar bone and a longer newly formed cementum were observed in the BDNF/HMW-HA group than in the HMW-HA group, suggesting that HMW-HA assists the regenerative capacity of BDNF, although HMW-HA itself does not enhance periodontal tissue regeneration. Neither the poly (lactic-co-glycolic acid) group nor the BDNF/poly (lactic-co-glycolic acid) group enhanced periodontal tissue regeneration. In conclusion, HMW-HA is an adequate scaffold for the clinical application of BDNF.
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掲載誌名 |
Tissue engineering. Part A.
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巻 | 17巻
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号 | 7-8号
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開始ページ | 955
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終了ページ | 967
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出版年月日 | 2011
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出版者 | Mary Ann Liebert
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ISSN | 1937-3341
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NCID | |
出版者DOI | |
言語 |
英語
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NII資源タイプ |
学術雑誌論文
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広大資料タイプ |
学術雑誌論文
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DCMIタイプ | text
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フォーマット | application/pdf
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著者版フラグ | publisher
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権利情報 | This is a copy of an article published in the Tissue engineering, Part A. (c) 2011 Mary Ann Liebert, Inc.; Tissue engineering, Part A. is available online at: http://online.liebertpub.com.
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関連情報URL(IsVersionOf) | http://dx.doi.org/10.1089/ten.TEA.2010.0070
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部局名 |
医歯薬学総合研究科
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