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ID 23280
本文ファイル
著者
Mishima, Takaaki
Sakamoto, Makoto
Sugiyama, Minako
Matsunaga, Satoshi
キーワード
muscle fatigue
structural modification
in vitro measurement
cation regulation protein
oxidative potential
NDC
生物科学・一般生物学
抄録(英)
Time-dependent changes in sarcoplasmic reticulum (SR) Ca2+-handling and Na+-K+-ATPase activity, as assessed in vitro, were investigated in the superficial (GS) and deep regions (GD) of rat gastrocnemius muscles undergoing short-term (up to 30 min) electrical stimulation. There was a rapid and progressive loss of force output during the first 5 min of stimulation. For GS, significant depressions (P < 0.05) in SR Ca2+-uptake rate and Ca2+-ATPase activity were observed during only the first 1 min. No further reductions occurred with stimulation time. SR Ca2+-release rate was significantly (P < 0.05) decreased at 3 min. For GD, significant reductions (P <0.05) in Ca2+-uptake rate, Ca2+-release rate and Ca2+-ATPase activity were manifested after 3, 5 and 5 min, respectively. A decay in Na+-K+-ATPase activity was found only in 1-min stimulated GD and 30-min stimulated GS. After 30 min, the depressed functions reverted to resting levels in GD but not in GS. The alterations in any variables examined were not parallel with changes in force output. These results suggest that, at least under the conditions used in this study, in vivo disruptions in cation regulation mediated by vigorous contractile activity would be attributable primarily to events other than structural alterations to the respective proteins.
掲載誌名
Pflügers Archiv : European Journal of Physiology
456巻
3号
開始ページ
601
終了ページ
609
出版年月日
2008-06
出版者
Springer-Verlag
ISSN
0031-6768
NCID
出版者DOI
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
author
権利情報
Copyright (c) 2007 Springer-Verlag
関連情報
The original version is abailable at www.springerlink.com
関連情報URL(IsVersionOf)
http://dx.doi.org/10.1007/s00424-007-0427-8
部局名
総合科学研究科
生物圏科学研究科