The inhibition of the nuclear transport of caspase-7 by its prodomain
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ID | 20660 |
本文ファイル | |
著者 | |
キーワード | caspase
nuclear localization
prodomain
apoptosis
cytotoxic activity
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NDC |
生物科学・一般生物学
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抄録(英) | Apoptosis is a major form of cell death, characterized by a series of the morphological changes induced by cleaving cytoplasmic and nuclear proteins via active caspases. The data presented here show, by the fluorescence microscopic and the immunoblotting analyses, that a prodomain of caspase-7 inhibits its nuclear translocation and apoptosis-inducing activity. This nuclear localization is dependent on the presence of a basic tetrapeptide that is conserved in mammalian and Xenopus caspase-7 and that is located downstream of a cleavage site between a prodomain and a catalytic protease domain. Furthermore, an attachment of caspase-7 prodomain (31 amino acids) represses the nuclear transport of a fusion protein of a heterologous protein and the caspase-7 nuclear localization signal (19 amino acids), suggesting that the inhibition of the nuclear localization by the prodomain is mediated by the interaction of these short peptides.
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掲載誌名 |
Biochemical and Biophysical Research Communications
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巻 | 291巻
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号 | 1号
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開始ページ | 79
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終了ページ | 84
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出版年月日 | 2002-02-15
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出版者 | Elsevier
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ISSN | 0006-291X
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NCID | |
出版者DOI | |
言語 |
英語
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NII資源タイプ |
学術雑誌論文
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広大資料タイプ |
学術雑誌論文
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DCMIタイプ | text
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フォーマット | application/pdf
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著者版フラグ | author
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権利情報 | Copyright (c) 2002 Elsevier Science (USA).
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関連情報URL | |
部局名 |
理学研究科
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