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ID 48606
本文ファイル
著者
Fujii, Sotaro 大学院生物圏科学研究科 広大研究者総覧
Oki, Hiroya
Kawahara, Kazuki
Yamane, Daisuke 大学院生物圏科学研究科
Yamanaka, Masaru
Maruno, Takahiro
Kobayashi, Yuji
Masanari, Misa 大学院生物圏科学研究科
Wakai, Satoshi
Nishihara, Hirofumi
Ohkubo, Tadayasu
Sambongi, Yoshihiro 大学院生物圏科学研究科 広大研究者総覧
キーワード
protein stability
cytochrome c
thermophile
protein structure
mutagenesis
ligand-binding
抄録(英)
Thermophilic Hydrogenophilus thermoluteolus cytochrome c0 (PHCP) exhibits higher thermal stability than a mesophilic counterpart, Allochromatium vinosum cytochrome c0 (AVCP), which has a homo-dimeric structure and ligand-binding ability. To understand the thermal stability mechanism and ligand-binding ability of the thermally stable PHCP protein, the crystal structure of PHCP was first determined. It formed a homo-dimeric structure, the main chain root mean square deviation (rmsd) value between PHCP and AVCP being 0.65 A ° . In the PHCP structure, six specific residues appeared to strengthen the heme-related and subunit–subunit interactions, which were not conserved in the AVCP structure. PHCP variants having altered subunit–subunit interactions were more severely destabilized than ones having altered heme-related interactions. The PHCP structure further revealed a ligand-binding channel and a penta-coordinated heme, as observed in the AVCP protein. A spectroscopic study clearly showed that some ligands were bound to the PHCP protein. It is concluded that the dimeric PHCP from the thermophile is effectively stabilized through heme-related and subunit–subunit interactions with conservation of the ligand-binding ability.
内容記述
This work was performed under the Cooperative Research Program of the “Network Joint Research Center for Materials and Devices”.
掲載誌名
Protein Science
26巻
4号
開始ページ
737
終了ページ
748
出版年月日
2017-03-06
出版者
Wiley
ISSN
0961-8368
1469-896X
出版者DOI
PubMedID
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
publisher
権利情報
© 2017 The Authors Protein Science published by Wiley Periodicals, Inc. on behalf of The Protein Society. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
関連情報URL
部局名
生物圏科学研究科