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ID 17064
本文ファイル
著者
Sasabe, Eri
Miyazaki, Kazuko
Fujimotoa, Katsumi
Kato, Yukio
キーワード
Dec1
Stra13
Bhlhb2
DEC2
mesenchymal stem cell
osteogenesis
adipogenesis
NDC
医学
抄録(英)
We recently reported that forced expression of basic helix-loop-helix transcription factor Dec1 accelerated chondrogenic differentiation of mesenchymal stem cells (MSC) in pellet cultures (M. Shen, E. Yoshida, W. Yan, T. Kawamoto, K. Suardita, Y. Koyano, K. Fujimoto, M. Noshiro and Y. Kato, 2002. J. Biol. Chem. 277:50112-50120). Since MSC have multilineage differentiation potential, we investigated the roles of Dec1 in osteogenic and adipogenic differentiation of human bone marrow-derived MSC. After osteogenic induction of MSC in medium containing dexamethasone, β- glycerophosphate, and ascorbic acid, Dec1 expression gradually increased from day 5 to day 14, while expression levels of Dec1 mRNA markedly decreased on days 3 and 7 after adipogenic induction. Infection with adenovirus expressing Dec1 raised mRNA levels of several bone characteristic molecules such as osteopontin, PTH receptor and alkaline phosphatase, even in the absence of the osteogenic-induction medium, although it had little effect on Runx2 expression or calcification. In the osteogenic-induction medium, Dec1 overexpression enhanced the expression of osteopontin and alkaline phosphatase and induced matrix calcification. Knockdown of Dec1 with siRNA suppressed the expression of osteoblastic phenotype by the induced MSC. Using MSC cultures, we also confirmed that forced expression of Dec1 suppressed adipogenic differentiation. These findings suggest that Dec1 modulates osteogenic differentiation of MSC by inducing the expression of several, but not all, bone-related genes.
掲載誌名
European Journal of Cell Biology
85巻
5号
開始ページ
423
終了ページ
431
出版年月日
2006-03-03
出版者
Elsevier
ISSN
0171-9335
NCID
出版者DOI
PubMedID
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
author
権利情報
Copyright (c) 2006 Elsevier GmbH.
関連情報URL
部局名
医歯薬学総合研究科