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ID 25930
本文ファイル
著者
Makihira, Seicho
Kawahara, Yumi
Mine, Yuichi
キーワード
Osteoblast cells
Osteoclast cells
3D clinostat
Microgravity
RANKL
OPG
NDC
医学
抄録(英)
Mechanical unloading conditions result in decreases in bone mineral density and quantity, which may be partly attributed to an imbalance in bone formation and resorption. To investigate the effect of mechanical unloading on osteoblast and osteoclast differentiation, and the expression of RANKL and OPG genes in osteoblasts, we used a three-dimensional (3D) clinostat system simulating microgravity to culture MC3T3-E1 and RAW264.7 cells. Long-term exposure (7 days) of MC3T3-E1 cells to microgravity in the 3D clinostat inhibited the expression of Runx2, Osterix, type I collagen αI chain, RANKL and OPG genes. Similarly, 3D clinostat exposure inhibited the enhancement of β3-integrin gene expression, which normally induced by sRANKL stimulation in RAW264.7 cells. These results, taken together, demonstrate that long-term 3D clinostat exposure inhibits the differentiation of MC3T3-E1 cells together with suppression of RANKL and OPG gene expression, as well as the RANKL-dependent cellular fusion of RAW264.7 cells, suggesting that long-term mechanical unloading suppresses bone formation and resorption.
掲載誌名
Cell Biology International
32巻
9号
開始ページ
1176
終了ページ
1181
出版年月日
2008-09
出版者
Academic Press Inc.
Elsevier Science
ISSN
1065-6995
NCID
出版者DOI
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
author
権利情報
Copyright (c) 2008 International Federation for Cell Biology Published by Elsevier Ltd.
関連情報URL
部局名
医歯薬学総合研究科