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ID 48611
本文ファイル
著者
Yukawa, Masashi 大学院先端物質科学研究科 広大研究者総覧
Kawakami, Tomoki
Okazaki, Masaki
Kume, Kazunori 大学院先端物質科学研究科 広大研究者総覧
Ngang, Heok Tang
Toda, Takashi 大学院先端物質科学研究科 広大研究者総覧
抄録(英)
Accurate chromosome segregation relies on the bipolar mitotic spindle. In many eukaryotes, spindle formation is driven by the plus-end–directed motor kinesin-5 that generates outward force to establish spindle bipolarity. Its inhibition leads to the emergence of monopolar spindles with mitotic arrest. Intriguingly, simultaneous inactivation of the minus-end–directed motor kinesin-14 restores spindle bipolarity in many systems. Here we show that in fission yeast, three independent pathways contribute to spindle bipolarity in the absence of kinesin-5/Cut7 and kinesin-14/Pkl1. One is kinesin-6/Klp9 that engages with spindle elongation once short bipolar spindles assemble. Klp9 also ensures the medial positioning of anaphase spindles to prevent unequal chromosome segregation. Another is the Alp7/TACC-Alp14/TOG microtubule polymerase complex. Temperature-sensitive alp7cut7pkl1 mutants are arrested with either monopolar or very short spindles. Forced targeting of Alp14 to the spindle pole body is sufficient to render alp7cut7pkl1 triply deleted cells viable and promote spindle assembly, indicating that Alp14-mediated microtubule polymerization from the nuclear face of the spindle pole body could generate outward force in place of Cut7 during early mitosis. The third pathway involves the Ase1/PRC1 microtubule cross-linker that stabilizes antiparallel microtubules. Our study, therefore, unveils multifaceted interplay among kinesin-dependent and -independent pathways leading to mitotic bipolar spindle assembly.
内容記述
This work was supported by the Japan Society for the Promotion of Science (JSPS) (KAKENHI Scientific Research [A] 16H02503 to T.T., a Challenging Exploratory Research grant 16K14672 to T.T., Scientific Research [C] 16K07694 to M.Y.), the Naito Foundation (T.T.), and the Uehara Memorial Foundation (T.T.).
掲載誌名
Molecular Biology of the Cell
28巻
25号
開始ページ
3647
終了ページ
3659
出版年月日
2017-12-01
出版者
The American Society for Cell Biology
ISSN
1059-1524
1939-4586
出版者DOI
PubMedID
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
publisher
権利情報
© 2017 Yukawa et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).
関連情報URL
部局名
先端物質科学研究科