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ID 25579
本文ファイル
著者
Tanaka, Nobuaki
Ueki, Masashi
Lin, Y. Y.
Ohkuma, S
Kamiya, Takashi
Tanaka, Eiji
Tanne, Kazuo
キーワード
Runt-related gene 2 (Runx2)
Hyaluronan (HA)
Chondrocyte
Differentiation
HAoligo
NDC
医学
抄録(英)
During endochondral ossification, the production of hyaluronan (HA) is strictly and selectively regulated by chondrocytes, with a temporal peak at the hypertrophic stage. This study was conducted to clarify the effects of HA on expression and activity of runt-related gene 2 (Runx2), a potent transcription factor for chondrocyte differentiation in hypertrophic chondrocytes. Immature chondrocytes from an ATDC5 cell line were cultured and differentiated in DMEM/Ham's F12 with pre-defined supplements. Using real-time PCR, the gene expressions of type II collagen, MMP-13, HAS2, and Runx2 in cultured chondrocytes were analysed from days 0 to 18 of cell differentiation. The activity and expression of Runx2 in hypertrophic chondrocytes were analysed after the treatment with HA oligosaccharide (HAoligo) using AML-3/Runx2 binding, real-time PCR and Western blot analysis. The effects of pre-incubation of anti-CD44 antibody on Runx2 expression were also examined. Expression of type X collagen and Runx2 mRNAs reached a maximum at the terminal differentiation of chondrocytes. The activity and expression of Runx2 was significantly inhibited in hypertrophic chondrocytes treated with HAoligo compared to the untreated controls. High molecular weight-HA did not affect the expression or activity of Runx2. The expression of Runx2 mRNA was significantly decreased in hypertrophic chondrocytes treated with anti-CD44 antibody. These results suggest that HAoligo may affect the terminal differentiation of chondrocytes during the endochondral ossification by inhibiting the expression and activity of Runx2.
掲載誌名
Archives of Oral Biology
53巻
5号
開始ページ
478
終了ページ
487
出版年月日
2008-05
出版者
Elsevier Ltd
ISSN
0003-9969
NCID
出版者DOI
PubMedID
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
author
権利情報
Copyright (c) 2007 Elsevier Ltd
関連情報URL(IsVersionOf)
http://dx.doi.org/10.1016/j.archoralbio.2007.12.007
部局名
医歯薬学総合研究科