このエントリーをはてなブックマークに追加
ID 49780
本文ファイル
著者
Arfan, Ahmad
KURIHARA, Sho 大学院医系科学研究科(医) 広大研究者総覧
KOJIMA, Masato
KIMURA, Shingo
KANAWA, Masami 自然科学研究支援開発センター 広大研究者総覧
SOTOMARU, Yusuke 自然科学研究支援開発センター 広大研究者総覧
HIYAMA, Eiso 自然科学研究支援開発センター 広大研究者総覧
キーワード
Neuroblastoma
microRNA
Prognosis
NGS
抄録(英)
Efforts to identify biomarkers for neuroblastoma (NB) have been ongoing, but no definite biomarker has been identified in peripheral blood. We proposed the use of plasma exosomal miRNAs as biomarkers of unfavorable NB patient outcomes. Exosomal miRNAs isolated from 31 plasma and 37 tissue samples, many from the same NB patients, were sequenced using a next-generation sequencing instrument. We analyzed the correlation between miRNA expression levels in plasma and tissue samples with International Neuroblastoma Risk Group staging system (INRGSS) outcome and MYCN status. We chose differentially expressed miRNAs with similar expression patterns in plasma and tissue samples in each of the three analysis groups and combined those miRNAs to find the optimal combination with the potential to be considered as a biomarker. MicroRNA-92a-3p was found to be significantly upregulated in deceased patients (p = 0.017), miR-375 was upregulated in INRGSS stage M patients (p = 0.002), and plasma miR-92a-3p and miR-99a-5 levels were upregulated in patients with MYCN amplification (p = 0.007 and 0.006). The combination of miR-92a-3p, miR-375, and miR-99a-5p levels was shown to be a statistically significant predictor of NB patient outcomes (AUC = 0.726, p = 0.001, 95% CI = 0.612–0.841, sensitivity = 77%, specificity = 56.7%). Thus, the combination of miR-92a3p, miR-375, and miR-99a-5p may potentially be used as a biomarker for unfavorable NB patient outcomes. However, further validation is required in a larger number of NB patients.
内容記述
This research was partially supported by a Grant-in-Aid for Scientific Research (A) (No.19H0105600) from the Ministry of Education, Culture, Sports, Science and Technology.
掲載誌名
Hiroshima Journal of Medical Sciences
69巻
2号
開始ページ
39
終了ページ
45
出版年月日
2020-06-30
出版者
Hiroshima University Medical Press
ISSN
0018-2052
2433-7668
NCID
出版者DOI
言語
英語
NII資源タイプ
紀要論文
広大資料タイプ
学内刊行物(紀要等)
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
publisher
権利情報
Copyright (c) 2020 Hiroshima University Medical Press
関連情報URL
部局名
医系科学研究科
自然科学研究支援開発センター
他の一覧