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ID 21464
本文ファイル
著者
Kuratsune, Masatoshi
Hirai, Takayuki
Kiribayashi, Kei
Yokoyama, Yukio
Arakawa, Tetsuji
Yorioka, Noriaki
キーワード
fibrosis
STAT3
UUO
NDC
医学
抄録(英)
Background: In vitro studies suggest that the signal transducer and activator of transcription (STAT) plays a critical role in renal fibrosis. However, the process of STAT activation in vivo remains unclear. This study in rats aimed to localize STAT3 activation within the kidney and examine the in vivo relationship between STAT3 activation and renal fibrosis Methods: Unilateral ureteral obstruction (UUO) was induced in the rats and the kidneys examined 3 or 7 days after obstruction. Activation of STAT3 in western blot and immunohistochemical analyses was identified by the phosphorylated form of STAT3 (pSTAT3). Results: Myofibroblasts were identified by alpha-smooth muscle actin expression and were upregulated in obstructed kidneys. pSTAT3 was localized mainly in tubular epithelial cells of collecting ducts in normal and obstructed kidneys and interstitial cells in obstructed kidneys. After UUO, western blotting showed a fourfold increase in pSTAT3, with a peak at day 7. Immunostaining showed a sixfold increase in pSTAT3 at day 7 in tubular epithelial cells and a 2500-fold increase at day 7 in interstitial cells. Conclusion: STAT3 was activated in rat tubular epithelial cells and myofibroblasts after UUO, suggesting that STAT3 may contribute to the progression of interstitial fibrosis.
掲載誌名
Nephrology
12巻
6号
開始ページ
565
終了ページ
571
出版年月日
2007-12
出版者
Blackwell Publishing
ISSN
1320-5358
NCID
出版者DOI
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
author
権利情報
Copyright(c) 2007 Blackwell Publishing
関連情報
The definite version is avairable at www.blackwell-synergy.com
関連情報URL(IsVersionOf)
http://dx.doi.org/10.1111/j.1440-1797.2007.00881.x
部局名
医歯薬学総合研究科