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ID 14652
本文ファイル
著者
Shitashiro, Maiko
Tanaka, Hirohide
Hong, Chang Soo
Takiguchi, Noboru
Ohtake, Hisao
Kato, Junichi
キーワード
chemotaxis
chemosensory protein
trichloroethylene
methyl-acceptiong
chemotaxis protein
pseudomonas aeruginosa
NDC
生物科学・一般生物学
内容記述
The involvement of the chemotaxis gene cluster 1 (cheYZABW) and cheR in repellent responses of Pseudomonas aeruginosa to trichloroethylene (TCE) is described and three methyl-accepting chemotaxis proteins (MCPs) for TCE are identified. TCE chemotaxis assays of a number of deletion-insertion mutants of P. aeruginosa PAO1 revealed that the chemotaxis gene cluster 1 and cheR are required for negative chemotaxis to TCE. Mutant strains which contained deletions in pctA, pctB and pctC showed decreased responses to TCE. The pctA, pctB and pctC genes have been reported to encode MCPs for amino acids [K. Taguchi et al., Microbiology, 143, 3223–3229 (2000)]. The pctA mutation more severely impaired chemotactic responses to TCE than did those of pctB and pctC, suggesting that PctA is the major MCP for TCE among the three MCPs. The pctA, pctB and pctC mutant strains showed decreased responses to chloroform and methylthiocyanate. This result demonstrates that PctA, PctB and PctC are also involved in repellent responses to chloroform and methylthiocyanate.
掲載誌名
Journal of Bioscience and Bioengineering
99巻
4号
開始ページ
396
終了ページ
402
出版年月日
2005-01
出版者
日本生物工学会
ISSN
1389-1723
NCID
出版者DOI
PubMedID
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
author
権利情報
Copyright (c) The Society for Biotechnology, Japan
関連情報URL(IsVersionOf)
http://dx.doi.org/10.1263/jbb.99.396
部局名
先端物質科学研究科