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ID 14650
本文ファイル
著者
Na, Kyung-su
Takiguchi, Noboru
Ikeda, Tsukasa
Ohtake, Hisao
Kato, Junichi
キーワード
solvent-tolerant bacterium
rhodococcus opacus
benzene-oxidizing bacterium
two-phase system
benzene dioxygenase gene mutation
NDC
生物科学・一般生物学
抄録(英)
Twenty-two benzene-utilizing bacteria were isolated from soil samples. Among them, three isolates were highly tolerant to benzene. They grew on benzene when liquid benzene was added to the basal salt medium at 10–90% (v/v). Taxonomical analysis identified the benzene-tolerant isolates as Rhodococcus opacus. One of the benzene-tolerant isolates, designated B-4, could utilize many aromatic and aliphatic hydrocarbons including benzene, toluene, styrene, xylene, ethylbenzene, propylbenzene, n-octane and n-decane as sole sources of carbon and energy. Strain B-4 grew well in the presence of 10% (v/v) organic solvents that it was capable of using as growth substrates. Genetic analysis revealed the benzene dioxygenase pathway is involved in benzene catabolism in strain B-4. A deletion-insertion mutant defective in the benzene dioxygenase large and small subunits genes (bnzA1 and bnzA2) was as tolerant to organic solvents as the wild-type strain B-4, suggesting that utilization or degradation of organic solvents is not essential for the organic solvent tolerance of R. opacus B-4.
掲載誌名
Journal of Bioscience and Bioengineering
99巻
4号
開始ページ
378
終了ページ
382
出版年月日
2005-01
出版者
日本生物工学会
ISSN
1389-1723
NCID
出版者DOI
PubMedID
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
author
権利情報
Copyright (c) 2005 The Society for Biotechnology, Japan
関連情報URL(IsVersionOf)
http://dx.doi.org/10.1263/jbb.99.378
部局名
先端物質科学研究科