In this study, the effects of Niko Niko Room's educational support program perceived by university students, elementary students, and guardians were compared. 21 university students, 21 elementary students, and 21 guardians who took part in the educational support program held in 2011 answered questionnaires measuring ability of university students, degrees of satisfactions, and degrees of interests. Comparisons between university students and elementary students, between university students and guardians, between elementary students and guardians, and among university students, elementary students, and guardians were made. University students tended to underestimate their educational ability, compared to elementary students. Overall, university students, elementary students, and guardians positively evaluated the effects of the educational support program.