In this study, the effects of Niko Niko Room's educational support program perceived by university students, school children, and guardians were compared. 22 university students, 22 school children, and 22 guardians who took part in the educational support program held in 2010 answered questionnaires measuring ability of university students, degrees of satisfactions, and degrees of interests. Comparisons between university students and school children, between university students and guardians, between school children and guardians, and among university students, school children, and guardians were made. University students tended to underestimate their educational ability, compared to school children. Overall, university students, school children, and guardians positively evaluated the effects of the educational support program.