Analysis of Mec Regulator Genes in Clinical Methicillin-resistant Staphylococcus aureus Isolates according to the Production of Coagulase, Types of Enterotoxin, and Toxic Shock Syndrome Toxin-1

アクセス数 : 1111
ダウンロード数 : 115

今月のアクセス数 : 9
今月のダウンロード数 : 6
ファイル情報(添付)
HiroshimaJMedSci_48_49.pdf 857 KB 種類 : 全文
タイトル ( eng )
Analysis of Mec Regulator Genes in Clinical Methicillin-resistant Staphylococcus aureus Isolates according to the Production of Coagulase, Types of Enterotoxin, and Toxic Shock Syndrome Toxin-1
作成者
Santo Takahiro
Takesue Yoshio
Matsuura Yuichiro
Yokoyama Takashi
収録物名
Hiroshima Journal of Medical Sciences
48
2
開始ページ 49
終了ページ 56
収録物識別子
[PISSN] 0018-2052
[EISSN] 2433-7668
[NCID] AA00664312
抄録
The intrinsic resistance of methicillin-resistant Staphylococcus aureus (MRSA) is frequently explained by the production of an additional penicillin-binding protein (PEP), which is encoded by the mecA gene. The mec regulator genes, mecR1 and mecI, was identified in mecA-carrying Staphylococcus aureus N315. Between February and March, 1993, 179 clinical MRSA isolates were collected from institutions in Hiroshima prefecture. According to serological types of coagulase, enterotoxins, and toxic shock syndrome toxin-1 (TSST-1) productions, these strains were classified into 6 groups. In 53 strains chosen from all groups, mec regulatory gene distributions were divided into two groups; one with whole regulatory genes and another with the lacking region, including 3'-partial region of the mecR1 gene and mecI gene. This same deletion was detected across the different groups, suggesting that the deletion occurred at the ancestral strain before branching according to coagulase or enterotoxin productions. The strains with this lacking region showed a high-level of resistance to methicillin, while the strains with whole regulatory genes consisted of low and high levels of resistant strains. The highly resistant strains with whole regulatory genes were found to harbor a point mutation in the mecI gene. The basal levels of mecA gene transcription were elevated in the strains with the lacking region or the mecI point mutations. These data suggest that deletion or mutation of the mecI gene, the repressor on the mecA gene, might play an important role in methicillin resistance in clinical isolates of MRSA.
著者キーワード
Methicillin-resistant Staphylococcus aureus (MRSA)
Mec regulator genes
Enterotoxins
Toxic shock syndrome toxin-I (TSST-1)
内容記述
This work was supported by a Grant-in-Aid for Cancer Research from the Ministry of Education, Science and Culture.
NDC分類
医学 [ 490 ]
言語
英語
資源タイプ 紀要論文
出版者
Hiroshima University Medical Press
発行日 1999-06
出版タイプ Version of Record(出版社版。早期公開を含む)
アクセス権 オープンアクセス
収録物識別子
[ISSN] 0018-2052
[NCID] AA00664312