Mizoribine Suppresses the Progression of Experimental Peritoneal Fibrosis in a Rat Model

Nephron Experimental Nephrology 112 巻 2 号 E59-E69 頁 2009 発行
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タイトル ( eng )
Mizoribine Suppresses the Progression of Experimental Peritoneal Fibrosis in a Rat Model
作成者
Takahashi Shunsuke
Taniguchi Yoshihiko
Arakawa Tetsuji
Kawai Toru
Ito Takafumi
Yorioka Noriaki
収録物名
Nephron Experimental Nephrology
112
2
開始ページ E59
終了ページ E69
抄録
Background/Aims: Peritoneal fibrosis is a serious complication of peritoneal dialysis (PD). It has been reported that administration of mizoribine, an effective immunosuppressant, ameliorated renal fibrosis in a rat model of unilateral ureteral obstruction. We therefore examined the effects of mizoribine in an experimental model of peritoneal fibrosis. Methods: 24 rats were given a daily intraperitoneal injection of chlorhexidine gluconate and ethanol dissolved in saline. The rats were divided into three groups (n = 8 per group) that received either vehicle or mizoribine at a dose of 2 or 8 mg/kg once a day. 28 days after the start of the treatments the rats were sacrificed and peritoneal tissue samples collected. Macrophage infiltration (ED1), myofibroblast accumulation (alpha-smooth muscle actin (SMA)) and expression of type III collagen, transforming growth factor (TGF)-beta and monocyte chemotactic protein-1 (MCP-1) were examined by immunohistochemistry. Results: Mizoribine significantly suppressed submesothelial zone thickening and reduced macrophage infiltration. Mizoribine also reduced collagen III+ area and decreased the number of alpha-SMA(+), TGF-beta(+) and MCP-1(+) cells. The magnitude of the changes observed was dose-dependent. Conclusion: The administration of mizoribine prevented the progression of peritoneal fibrosis in this rat model. Mizoribine may represent a novel therapy for peritoneal sclerosis in patients undergoing long-term PD.
著者キーワード
Macrophage
Mizoribine
Peritoneal dialysis
Peritoneal fibrosis
Experimental peritoneal fibrosis
rat model
NDC分類
医学 [ 490 ]
言語
英語
資源タイプ 学術雑誌論文
出版者
Karger
発行日 2009
権利情報
Copyright (c) 2009 S. Karger AG, Basel
出版タイプ Author’s Original(十分な品質であるとして、著者から正式な査読に提出される版)
アクセス権 オープンアクセス
収録物識別子
[ISSN] 1660-2129
[DOI] 10.1159/000213896
[DOI] http://dx.doi.org/10.1159/000213896