Molecular characterization of atmospheric NO2-responsive germin-like proteins in azalea leaves

Biochemical and Biophysical Research Communications 377 巻 3 号 857-861 頁 2008-12-18 発行
アクセス数 : 804
ダウンロード数 : 210

今月のアクセス数 : 0
今月のダウンロード数 : 4
ファイル情報(添付)
タイトル ( eng )
Molecular characterization of atmospheric NO2-responsive germin-like proteins in azalea leaves
作成者
Kondo Komei
Yamada Keizo
Nakagawa Ayami
Morikawa Hiromichi
収録物名
Biochemical and Biophysical Research Communications
377
3
開始ページ 857
終了ページ 861
抄録
Atmospheric nitrogen dioxide (NO2) is an environmental oxidant that is removed through direct uptake by foliage, but plant responses to this highly reactive gas are not well understood at the molecular level. From NO2-exposed leaves of a woody azalea (Rhododendron mucronatum), we cloned two cDNAs (RmGLP1 and RmGLP2) for germin-like proteins (GLPs), a group of ubiquitous plant proteins that have been implicated in various plant physiological and developmental processes. Quantitative analysis of mRNA expression, together with immunoblotting data, showed that foliar exposure to NO2 caused a robust induction of these GLP-encoding genes. When produced in tobacco cell culture, recombinant RmGLP2 was secreted into the apoplast, where it exhibited superoxide dismutase activity. RmGLP1 and RmGLP2 represent the first examples of plant genes that are responsive to airborne NO2. These enzymes might have a potential role in extracellular defense mechanisms through attenuation of interactions between reactive nitrogen and oxygen species.
著者キーワード
Germin-like protein
GLP
nitrogen dioxide
nitric oxide
reactive nitrogen species
superoxide dismutase
reactive oxygen species
NDC分類
生物科学・一般生物学 [ 460 ]
言語
英語
資源タイプ 学術雑誌論文
出版者
Academic Press Inc.
Elsevier Science
発行日 2008-12-18
権利情報
Copyright (c) 2008 Elsevier Inc.
出版タイプ Author’s Original(十分な品質であるとして、著者から正式な査読に提出される版)
アクセス権 オープンアクセス
収録物識別子
[ISSN] 0006-291X
[DOI] 10.1016/j.cbpb.2008.07.007
[NCID] AA00564395
[DOI] http://dx.doi.org/10.1016/j.cbpb.2008.07.007