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ID 14885
file
creator
Tan, Hironobu
Okazaki, Koei
Kubota, Ichiro
Kamiryo, Tatsuyuki
Utiyama, Hiroyasu
NDC
Biology
abstract
We have sequenced the nucleotides of the gene POX18 that encodes PXP-18, a major peroxisomal polypeptide inducible by oleic acid in the yeast Candida tropicalis. POX18 had a single open reading frame of 127 amino acids. Some 330f the amino acid sequence of the predicted basic polypeptide (13805 Da), was identical to that of the nonspecific lipid-transfer protein (sterol carrier protein 2) from rat liver. PXP-18, purified to near homogeneity from isolated peroxisomes, had an amino-terminal sequence identical to that of the predicted polypeptide except for the initiator methionine, and had nonspecific lipid-transfer activity comparable to that of its mammalian equivalents. Unexpectedly, PXP-18 lacked the cysteine residue thought to be essential for the activity of this protein in mammals. RNA blot analysis showed that the POX18 gene was expressed exclusively in cells grown on oleic acid, suggesting that PXP-18 has a role in the β-oxidation of long-chain fatty acids. PXP-18 modulated acyl-coenzyme A oxidase activity at low pH.
journal title
European Journal of Biochemistry
volume
Volume 190
issue
Issue 1
start page
107
end page
122
date of issued
1990
publisher
Federation of European Biochemical Societies
issn
0014-2956
ncid
publisher doi
pubmed id
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
author
rights
Copyright (c) 1990 Federation of European Biochemical Societies
relation is version of URL
http://dx.doi.org/10.1111/j.1432-1033.1990.tb15552.x
department
Graduate School of Integrated Arts and Sciences