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ID 39963
file
creator
Kume, Satoshi
Matsuoka, Hiroki
Hamada, Mayuko
Satoh, Nori
Michibata, Hitoshi
subject
Ascidian
Vanadium
Microarray
Gene regulation
Glutathione
NDC
Biology
abstract
Ascidians are hyperaccumulators that have been studied in detail. Proteins and genes involved in the accumulation process have been identified, but regulation of gene expression related to vanadium accumulation remains unknown. To gain insights into the regulation of gene expression by vanadium in a genome-wide manner, we performed a comprehensive study on the effect of excess vanadium ions on a vanadium-rich ascidian, Ciona intestinalis, using a microarray. RT-PCR and enzyme activity assay were performed from the perspective of redox and accumulation of metal ions in each tissue. Glutathione metabolism-related proteins were significantly up-regulated by VIV treatment. Several genes involved in the transport of vanadium and protons, such as Nramp and V-ATPase, were significantly up-regulated by VIV treatment. We observed significant up-regulation of glutathione synthesis and degradation pathways in the intestine and branchial sac. In blood cells, expression of Ci-Vanabin4, glutathione reductase activity, glutathione levels, and vanadium concentration increased after VIV treatment. VIV treatment induced significant changes related to vanadium exclusion, seclusion, and redox pathways in the intestine and branchial sac. It also induced an enhancement of the vanadium reduction and accumulation cascade in blood cells. These differential responses in each tissue in the presence of excess vanadium ions suggest that vanadium accumulation and reduction may have regulatory functions. This is the first report on the gene regulation by the treatment of vanadium-rich ascidians with excess vanadium ions. It provided much information for the mechanism of regulation of gene expression related to vanadium accumulation.
description
This work was supported in part by Grants-in-Aid from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (#21570077 to H. M. and #20570070 to T. U.) and a grant from the Toray Science Foundation (03-4402 to T. U.).
journal title
BioMetals
volume
Volume 25
issue
Issue 5
start page
1037
end page
1050
date of issued
2012-10
publisher
Springer Netherlands
issn
0966-0844
ncid
publisher doi
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
author
rights
Copyright (c) Springer Science+Business Media, LLC. 2012
The final publication is available at Springer via http://dx.doi.org/10.1007/s10534-012-9569-z.
relation is version of URL
http://doi.org/10.1007/s10534-012-9569-z
department
Graduate School of Science