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ID 36286
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title alternative
多剤耐性緑膿菌のblaIMP-1インテグロンに存在する新規アミノグリコシドアセチルトランスフェラーゼAAC(6’)-Iagの同定と解析
creator
Kobayashi, Kanao
NDC
Medical sciences
abstract
In a continuing study from Dec 2006 to Apr 2008, we characterized nine multi-drug resistant Pseudomonas aeruginosa strains isolated from four patients in a ward at the Hiroshima University Hospital, Japan. Pulsed-field gel electrophoresis of SpeI-digested genomic DNAs from the isolates suggested the clonal expansion of a single strain; however, only one strain, NK0009, was found to produce metallo-β-lactamase. PCR and subsequent sequencing analysis indicated NK0009 possessed a novel class 1 integron, designated as In124, that carries an array of four gene cassettes: a novel aminoglycoside (AG) resistance gene, aac(6')-Iag, blaIMP-1, a truncated form of blaIMP-1, and a truncated form of aac(6')-Iag. The aac(6')-Iag encoded a 167-amino-acid protein that shows 40% identity with AAC(6')-Iz. Recombinant AAC(6')-Iag protein showed aminoglycoside 6'-N-acetyltransferase activity using thin-layer chromatography (TLC) and MS spectrometric analysis. Escherichia coli carrying aac(6')-Iag showed resistance to amikacin, arbekacin, dibekacin, isepamicin, kanamycin, sisomicin, and tobramycin; but not to gentamicin. A conjugation experiment and subsequent Southern hybridization with the gene probes for blaIMP-1 and aac(6')-Ig strongly suggested In124 is on a conjugal plasmid. Transconjugants acquired resistance to gentamicin and were resistant to virtually all AGs, suggesting that the In124 conjugal plasmid also possesses a gene conferring resistance to gentamicin.
date of created
2014-11-21
language
eng
nii type
Thesis or Dissertation
HU type
Doctoral Theses
DCMI type
text
format
application/pdf
text version
ETD
rights
Copyright(c) by Author
relation references
Kanao Kobayashi, Ikue Hayashi, Syuntaro Kouda, Fuminori Kato, Tamaki Fujiwara, Shizuo Kayama, Hideki Hirakawa, Hideyuki Itaha, Hiroki Ohge, Naomasa Gotoh, Tsuguru Usui, Akio Matsubara and Motoyuki Sugai; Identification and Characterization of a Novel aac(6')-Iag Associated with the blaIMP-1-Integron in a Multidrug-Resistant Pseudomonas aeruginosa; PLoS ONE 8(8): e70557. (doi: 10.1371/journal.pone.0070557)
relation references URL
http://dx.doi.org/10.1371/journal.pone.0070557
grantid
甲第6285号
degreeGrantor
広島大学(Hiroshima University)
degreename Ja
博士(医学)
degreename En
Philosophy in Medical Science
degreelevel
doctoral
date of granted
2014-02-27
department
Graduate School of Biomedical Science