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ID 48657
file
creator
Kunitake, Hirofumi
Hiramatsu, Takahiro
Kinashi, Haruyasu
subject
biosynthesis
genetic engineering
azoxyalkene
natural products
Streptomyces
abstract
Streptomyces rochei 7434AN4 predominantly produces lankacidin and lankamycin under normal culture conditions, suggesting that other biosynthetic gene clusters for secondary metabolites are silent. To exploit silent metabolites of strain 7434AN4, we constructed mutant KA57, a multiple disruptant of the transcriptional repressor gene srrB together with the biosynthesis genes for both antibiotics. Mutant KA57 accumulated a compound (KA57-A) with a strong UV absorption at 235 nm, which was not detected in the parent strain or other mutants. Various spectroscopic analyses revealed that KA57-A is an azoxyalkene compound of a molecular formula of C10H20N2O3 with the R configuration at C-2. Biosynthesis of KA57-A was also studied by feeding with labeled acetates, amino acids, and 1-hexylamine. The hexenyl moiety (C1’-C6’) was derived from fatty acid, while the 3-amino-butan-1,2-diol moiety (C1-C4) was derived from C-2 of acetate (C1) and serine (C2-C4). Incorporation of [1,1-2H2]1-hexylamine indicated that C1’-C2’ dehydrogenation occurs at the final step of biosynthesis.
description
アクセプト後にアブストラクト等変更あり。
This work was supported by a Grants‐in‐Aid for Scientific Research on Innovative Areas from the Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT), and a Noda Institute for Scientific Research Grant.
Supporting information for this articleis available on the WWW under http://dx.doi.org/10.1002/cbic.201500393.
journal title
CHEMBIOCHEM
volume
Volume 16
issue
Issue 15
start page
2237
end page
2243
date of issued
2015-10-12
publisher
Wiley‐VCH Verlag
issn
1439-4227
1439-7633
publisher doi
pubmed id
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
author
rights
This is the peer reviewed version of the following article: CHEMBIOCHEM. 2015 Oct 12;16(15):2237-2243, which has been published in final form at https://doi.org/10.1002/cbic.201500393. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
relation url
department
Graduate School of Advanced Sciences of Matter