このエントリーをはてなブックマークに追加
ID 48652
file
creator
Takahashi, Shiro
Sugiyama, Taiki
Shimomura, Mayuka
Kamada, Yoshihiro
Fujita, Kazutoshi
Nonomura, Norio
Miyoshi, Eiji
subject
fucosylated haptoglobin
gastroenterological cancer
metastatic prostate cancer
linkage of fucose
site-specific analysis
abstract
Fucosylation is an important type of glycosylation involved in cancer, and fucosylated proteins could be employed as cancer biomarkers. Previously, we reported that fucosylated N-glycans on haptoglobin in the sera of patients with pancreatic cancer were increased by lectin-ELISA and mass spectrometry analyses. However, an increase in fucosylated haptoglobin has been reported observed in various types of cancer. To ascertain if characteristic fucosylation is observed in each cancer type, we undertook site-specific analyses of N-glycans on haptoglobin in the sera of patients with five types of operable gastroenterological cancer (esophageal, gastric, colon, gallbladder, pancreatic), a non-gastroenterological cancer (prostate cancer) and normal controls using ODS column LC-ESI MS. Haptoglobin has four potential glycosylation sites (Asn184, Asn207, Asn211, Asn241). In all cancer samples, monofucosylated N-glycans were significantly increased at all glycosylation sites. Moreover, difucosylated N-glycans were detected at Asn 184, Asn207 and Asn241 in only cancer samples. Remarkable differences in N-glycan structure among cancer types were not observed. We next analyzed N-glycan alditols released from haptoglobin using graphitized carbon column LC-ESI MS to identify the linkage of fucosylation. Lewis-type and core-type fucosylated N-glycans were increased in gastroenterological cancer samples, but only core-type fucosylated N-glycan was relatively increased in prostate cancer samples. In metastatic prostate cancer, Lewis-type fucosylated N-glycan was also increased. These data suggest that the original tissue/cell producing fucosylated haptoglobin is different in each cancer type and linkage of fucosylation might be a clue of primary lesion, thereby enabling a differential diagnosis between gastroenterological cancers and non-gastroenterological cancers.
journal title
Glycoconjugate Journal
volume
Volume 33
issue
Issue 3
start page
471
end page
482
date of issued
2016-02-11
publisher
Springer Verlag
issn
0282-0080
1573-4986
publisher doi
pubmed id
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
author
rights
This is a post-peer-review, pre-copyedit version of an article published in Glycoconjugate Journal. The final authenticated version is available online at: https://doi.org/10.1007/s10719-016-9653-7
relation url
department
Graduate School of Advanced Sciences of Matter