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ID 51508
file
creator
Lamboley, Cedric R.
Lamb, Graham D.
subject
Oxidative stress
Muscle elasticity
Skinned fbre
Titin
Passive force
abstract
This study investigated the efect of S-glutathionylation on passive force in skeletal muscle fbres, to determine whether activity-related redox reactions could modulate the passive force properties of muscle. Mechanically-skinned fbres were freshly obtained from human and rat muscle, setting sarcomere length (SL) by laser difraction. Larger stretches were required to produce passive force in human fbres compared to rat fbres, but there were no fbre-type diferences in either species. When fbres were exposed to glutathione disulfde (GSSG; 20 mM, 15 min) whilst stretched (at a SL where passive force reached~20% of maximal Ca2+-activated force, denoted as SL20 % max), passive force was subsequently decreased at all SLs in both type I and type II fbres of rat and human (e.g., passive force at SL20 % max decreased by 12 to 25%). This decrease was fully reversed by subsequent reducing treatment with dithiothreitol (DTT; 10 mM for 10 min). If freshly skinned fbres were initially treated with DTT, there was an increase in passive force in type II fbres (by 10±3% and 9±2% in rat and human fbres, respectively), but not in type I fbres. These results indicate that (i) S-glutathionylation, presumably in titin, causes a decrease in passive force in skeletal muscle fbres, but the reduction is relatively smaller than that reported in cardiac muscle, (ii) in rested muscle in vivo, there appears to be some level of reversible oxidative modifcation, probably involving S-glutathionylation of titin, in type II fbres, but not in type I fbres.
description
We thank the National Health and Medical Research Council of Australia for financial support (Grant No. 1085331).
journal title
Journal of Muscle Research and Cell Motility
volume
Volume 41
start page
239
end page
250
date of issued
2019-11-02
publisher
Springer
issn
0142-4319
1573-2657
publisher doi
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
author
rights
This is not the published version. Please cite only the published version. この論文は出版社版ではありません。引用の際には出版社版をご確認、ご利用ください。
relation url
department
Graduate School of Humanities and Social Sciences