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ID 49994
file
creator
SAKAGAMI, Kenichi
NAMBA, Akira
TANAKA, Kohtaro
OSAKI, Toshihide
ONISHI, Tetsuya
SONE, Yoshiyuki
KINOSHITA, Masaharu
ORITA, Kunzo
subject
Active enhancement
Rat cardiac allograft
MLR inhibitory factors
NDC
Medical sciences
abstract
In an attempt to induce the active enhancement of the cardiac allografts, recipient rats were injected intravenously with 1 x 107 donor-specific spleen cells or an equal number of their T or B cell subpopulations on each of 7 days before transplantation. Mean survival times (MST) in the group pretreated with donor-specific spleen cells (MST 27.5 ± 7.8 days) and the group pretreated with donor-specific B cells (MST 37.5 ± 15.5 days) were significantly prolonged (p<0.01) compared with the untreated control (MST 11.6 ± 2.0 days) and the group pretreated with donor-specific T cells. To investigate the mechanisms of the beneficial effect of donor-specific B cells on rat cardiac allografts, inhibition assay of mixed lymphocyte reaction (MLR) were carried out by the addition of recipient rat sera or spleen cells harvested 7 days following the intravenous administration of donor-specific spleen cells or their T and B cell subpopulations. Recipient rat sera harvested 7 days following the intravenous administration of donor-specific B cells showed significant inhibition of MLR; this inhibition was correlated to the prolonged survival of histoincompatible rat cardiac allografts. In contrast, MLR suppressor cells could not be detected in any experimental group. Thus, donor-specific B cell given 7 days before transplantation may possibly have beneficial effect on rat cardiac allografts and MLR inhibition induced by the intravenous administration of donor-specific B cells may be essential for prolonged allograft survival.
journal title
Hiroshima Journal of Medical Sciences
volume
Volume 36
issue
Issue 3
start page
295
end page
298
date of issued
1987-09
publisher
Hiroshima University Medical Press
issn
0018-2052
ncid
pubmed id
language
eng
nii type
Departmental Bulletin Paper
HU type
Departmental Bulletin Papers
DCMI type
text
format
application/pdf
text version
publisher
department
Graduate School of Biomedical Science
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